Demo serial dilution to final protein assay standard curve protocol?

[photocyte]

Recently, I've been working with a BSA serial dilution protocol (for measuring BSA standard curves via protein assays), which uses multiple SOPs (protocols in Google Doc format), excel documents (for calculation of tables, that are then embedded into the SOP Google Docs), and a Opentrons OT-2 python script.

There are then two follow up SOP protocols for measuring the actual standard curve, via the Bradford Assay, and the BCA Assay, both on a Tecan Spark plate reader (and the plate reader uses a separate instrument protocol/method). I even have Jupyter notebooks that take the excel file output from the Tecan Spark, and then parse the data & plot the standard curve.

There are a lot of moving parts in these protocols, it uses a variety of labware with both manual and automated manipulations, and I was just bitten (minorly) by the difficulty of propogating changes made in the early BSA SOP, all the way to the measurements & data analysis at the end. So I have some interest in a more robust LabOP protocol to represent this complex workflow.

This might be a good set of protocols plus sub-protocols, to use as a demo / stress test of LabOP in a complex context.

But, I don't have the time to contribute to making such a protocol/demo "out of whole cloth", but if folks are interested in asynchronously working on this, I could perhaps develop it with significant guidance.

See also:
#147