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Hi! Great tool, very convenient and easy to use! Just want to bring to your attention a potential problem. If chromosome names supplied to whitelist are not in the same order as the bam file, the labels are swapped in the plot. For example, using --whitelist X,Y the X chromosome appeared to have very low coverage, and the Y chromosome very high coverage - in a female sample that does not have a Y, so this seemed super weird! Samtools coverage showed low Y coverage and high X coverage, as anticipated. Y comes before X in my coordinate ordered bamfile, and running --whitelist Y,X recovers a plot that resembles what I'd expect based on samtools coverage output. The order of labels matches whitelist, but the order of which chromosome is plotted does not.
The text was updated successfully, but these errors were encountered:
Hey @bmsacchi, thanks for reporting this issue! 😃
I'll take a look when possible, but if you have an idea for a fix or feel like contributing, I would gladly review and accept a PR.
Hi! Great tool, very convenient and easy to use! Just want to bring to your attention a potential problem. If chromosome names supplied to whitelist are not in the same order as the bam file, the labels are swapped in the plot. For example, using --whitelist X,Y the X chromosome appeared to have very low coverage, and the Y chromosome very high coverage - in a female sample that does not have a Y, so this seemed super weird! Samtools coverage showed low Y coverage and high X coverage, as anticipated. Y comes before X in my coordinate ordered bamfile, and running --whitelist Y,X recovers a plot that resembles what I'd expect based on samtools coverage output. The order of labels matches whitelist, but the order of which chromosome is plotted does not.
The text was updated successfully, but these errors were encountered: