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Leaf-level Gas Exchange Methods Metadata Guide

This page provides guidelines and expectations for completion of the methods metadata template. Note that while defined vocabulary terms are suggested, most variables may be complete with free text entries if required.

The main purpose of this metadata collection will be to aid data search with some common discriminators. It is not meant to be a substitute for a complete experimental description. Additional information should be included as methods supplements tables, or in a methods text file.

Methods Metadata Content - Click links below for more details

* indicate required content

dataType* | additionalDataIncluded* | instrumentOutputStatus*

plantType* | leafType* | requiredProtocols*

mesophyllConductanceType | leafStatus* | leafAreaBasis*

leafAreaMethod* | kineticConstants | growthEnvironment*

experimentalManipulation* | plantAge* | leafAge*

canopyPosition* | canopyHeight* | lightExposure*

dataType

Metadata Element dataType
Required Recommended Optional required
Description Enter data type described by this methods metadata file. Refer to data types in documentation for definitions.
Format Controlled vocabulary or free text
Additional Instructions
Controlled vocabulary terms Refer to data types table for list.

additionalDataIncluded

Metadata Element additionalDataIncluded
Required Recommended Optional required
Description Indicate if additional data collected in-line during gas exchange is included. e.g. fluorescence, isotopic discrimination
Format Controlled vocabulary
Additional Instructions
Controlled vocabulary terms yes; no

instrumentOutputStatus

Metadata Element instrumentOutputStatus
Required Recommended Optional required
Description Indicate the highest quality status of complete instrument output included in the data package. Multiple versions may be included.
Format Controlled vocabulary
Additional Instructions 0 = data not available. 1 = direct instrument download, minimal quality control. 2 = complete quality control, files are verified to only contain valid data, or, invalid data is flagged. Measurement values are reasonable and within expected range. Area corrections are made where required. Time and dates verified as correct. User input errors corrected.
Controlled vocabulary terms 0; 1; 2

plantType

Metadata Element plantType
Required, Recommended, or Optional required
Description Photosynthetic pathway of measured plants.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms C3; C4; CAM

leafType

Metadata Element leafType
Required, Recommended, or Optional required
Description Describe the leaf type(s) measured.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms broadleaf; graminoid; needleleaf; phyllode

requiredProtocols

Metadata Element requiredProtocols
Required, Recommended, or Optional required
Description Details of measurement protocols. Refer to requiredProtocols for each data type for required protocol information. Can cite reference or other source if this information is recorded elsewhere.
Format Free text
Additional instructions
Controlled vocabulary terms

mesophyllConductanceType

Metadata Element mesophyllConductanceType
Required, Recommended, or Optional optional
Description Describe assumption or measurement of mesophyll conductance. Infinite mesophyll conductance assumes intercellular CO2 concentration (Ci) is equal to the CO2 concentration at the site of carboxylation inside the chloroplast (Cc).
Format Controlled vocabulary
Additional instructions
Controlled vocabulary terms assumed infinite; measured

leafStatus

Metadata Element leafStatus
Required, Recommended, or Optional required
Description Describe the leaf status during gas exchange measurement.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms attached leaf; attached leaf on excised branch; excised leaf

leafAreaBasis

Metadata Element leafAreaBasis
Required, Recommended, or Optional required
Description Describe the basis for measurement of leaf area. The default is "one-sided leaf area" which is typical for regular leaf types but there are several other alternatives, including in rare circumstances the use of mass or volume.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms one-sided area; hemi-surface area; projected area; total area; dry mass; volume

leafAreaMethod

Metadata Element leafAreaMethod
Required, Recommended, or Optional required
Description Describe the methods used for measurement of leaf area.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms single leaf fills chamber of known area; multiple leaves fill chamber of known area; geometrically determined; calculated using imaging software; measured using leaf area meter

kineticConstants

Metadata Element kineticConstants
Required, Recommended, or Optional optional
Description Citations and values for kinetic constants, and their temperature dependance.
Format Free text
Additional instructions
Controlled vocabulary terms

growthEnvironment

Metadata Element growthEnvironment
Required, Recommended, or Optional required
Description Describe the plant growth environment. Provide details in Methods.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms natural in ground; cultivated in ground; potted; glasshouse; controlled environment chamber; other chamber type; hydroponic

experimentalManipulation

Metadata Element experimentalManipulation
Required, Recommended, or Optional required
Description Indicate if any samples were subject to a manipulation. For naturally grown plants, or cultivated plants with standard fertilizer and pesticide application across all samples, select "none". Provide further treatment details in methodsSupplements tables.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms none; atmosphere; genetic; growth media; pesticide; light; nutrients; salinity; soil modification; source-sink; temperature; water availability

plantAge

Metadata Element plantAge
Required, Recommended, or Optional required
Description Age in appropriate units (days, months, years) or relative age category (e.g. seedling, germling, sapling, mature) or other classification relevant to experiment.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms. Include a methods supplements table if required.
Controlled vocabulary terms unknown; seedling; germling; sapling; mature

leafAge

Metadata Element leafAge
Required, Recommended, or Optional required
Description Age in appropriate units (days, months, years) or relative age category (e.g. young, mature, old) or other classification (e.g. most recent fully expanded, physiologically mature leaf; LPI classification).
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms. Include a methods supplements table if required.
Controlled vocabulary terms young; mature; old; unknown; multiple ages

canopyPosition

Metadata Element canopyPosition
Required, Recommended, or Optional required
Description Position of leaf samples within canopy.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms top of canopy; within canopy

canopyHeight

Metadata Element canopyHeight
Required, Recommended, or Optional required
Description Sample canopy position and canopy height or LAI recorded.
Format Controlled vocabulary
Additional instructions If yes, provide details in metadata supplements and/or data tables.
Controlled vocabulary terms yes; no

lightExposure

Metadata Element lightExposure
Required, Recommended, or Optional required
Description Light environment of leaf samples.
Format Controlled vocabulary or free text
Additional instructions List all that apply, use a semi-colon to delimit multiple terms.
Controlled vocabulary terms sunlit; shade