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<!DOCTYPE html>
<html>
<head>
<meta charset="utf-8">
<title>Strobe-enhanced microscopy stage</title>
<meta http-equiv="X-UA-Compatible" content="IE=edge">
<meta name="viewport" content="width=device-width, initial-scale=1">
<link rel="shortcut icon" href="./assets/favicon.ico" />
<link href="./static/style.css" rel="stylesheet">
<link href="./assets/style.css" rel="stylesheet">
</head>
<body>
<header class="site-header">
<div class="wrapper header-wrapper" >
<div class=header-text>
<h1 class="site-title">Strobe-enhanced microscopy stage</h1>
<p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p>
</div>
</div>
</header>
<div class="page-content">
<div>
<nav class="sidebar">
<a href="#" class="menu-icon">
<svg viewBox="0 0 18 15">
<path fill="#424242" d="M18,1.484c0,0.82-0.665,1.484-1.484,1.484H1.484C0.665,2.969,0,2.304,0,1.484l0,0C0,0.665,0.665,0,1.484,0 h15.031C17.335,0,18,0.665,18,1.484L18,1.484z"/>
<path fill="#424242" d="M18,7.516C18,8.335,17.335,9,16.516,9H1.484C0.665,9,0,8.335,0,7.516l0,0c0-0.82,0.665-1.484,1.484-1.484 h15.031C17.335,6.031,18,6.696,18,7.516L18,7.516z"/>
<path fill="#424242" d="M18,13.516C18,14.335,17.335,15,16.516,15H1.484C0.665,15,0,14.335,0,13.516l0,0 c0-0.82,0.665-1.484,1.484-1.484h15.031C17.335,12.031,18,12.696,18,13.516L18,13.516z"/>
</svg>
</a>
<ul class="nav-list">
<li>
<a class="navhome" href="./">
Strobe-enhanced microscopy stage
</a>
</li>
<li class="active">
<a class="active" href="./3-level-station.html">
Build the 3-level microscopy stage
</a>
<ul class="sub-nav-list">
<li>
<a class="not-active" href="./printing.html">
Print the plastic parts
</a>
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<li>
<a class="not-active" href="./laser-cutting.html">
Laser cut the acrylic parts
</a>
</li>
<li>
<a class="not-active" href="./assemble-focus-mechanism.html">
Assemble the focus mechanism
</a>
</li>
<li>
<a class="not-active" href="./basic-optics-module.html">
Assemble the basics optics module
</a>
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<a class="not-active" href="./attach-parts-to-top-plate.html">
Attach parts to the top plate
</a>
</li>
<li>
<a class="active" href="./attach-parts-to-middle-plate.html">
Attach parts to the middle plate
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-bottom-plate.html">
Attach parts to the bottom plate
</a>
</li>
<li>
<a class="not-active" href="./mount-optics.html">
Mount the optics
</a>
</li>
<li>
<a class="not-active" href="./wiring.html">
Mount the electronics
</a>
</li>
<li>
<a class="not-active" href="./illumination.html">
Assemble the illumination
</a>
</li>
<li>
<a class="not-active" href="./finished.html">
Assembly complete
</a>
</li>
</ul>
</li>
<li class="not-active">
<a class="not-active" href="./software/software-installation.html">
Software Installation
</a>
</li>
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<a class="not-active" href="./usage.html">
Use your Stage
</a>
</li>
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<a class="not-active" href="./troubleshooting.html">
Troubleshooting
</a>
</li>
<li class="not-active">
<a class="not-active" href="./variants_accesories.html">
Customisation and accessories
</a>
</li>
</ul>
</nav>
</div>
<div class="wrapper">
<h1 id="attach-parts-to-the-middle-plate">Attach parts to the middle plate</h1>
<div class="pagebom">
<h3 id="tool">Tools</h3>
<ul>
<li>1 <a Class="bom" href="parts/tools/2.5mmBallEndAllenKey.html">2.5mm Ball-end Allen key</a> </li>
</ul>
<h3 id="lasercutpart">Laser cut Parts</h3>
<ul>
<li>1 <a Class="bom" href="parts/materials/middle-plate.html">Middle plate</a> </li>
</ul>
<h3 id="mechanic">Mechanical Components</h3>
<ul>
<li>3 <a Class="bom" href="parts/mech/M3-10.html">M3x10mm cap head screw</a> </li>
<li>4 <a Class="bom" href="parts/mech/M3-15.html">M3x15mm cap head screw</a> </li>
</ul>
<h3 id="printedpart">Printed Parts</h3>
<ul>
<li>4 <a Class="bom" href="models/standoff-S.html">Standoff-S</a> </li>
</ul>
<h3 id="printedtool">Printed Tools</h3>
<ul>
<li>1 <a Class="bom" href="models/actuator-assembly-tools.html">Nut tool</a> </li>
</ul>
</div>
<p>You will also need the parts you assembled in previous sections.</p>
<h2 class="page-step" id="screw-into-the-focusing-assembly">Step 1: Screw into the focusing assembly</h2>
<ul>
<li>Attach the <a href="parts/materials/middle-plate.html">middle plate</a> to the <a href="assemble-focus-mechanism.html" title="">focusing mechanism</a> using three <a href="parts/mech/M3-10.html">M3x10mm cap head screws</a>.</li>
<li>Use a <a href="parts/tools/2.5mmBallEndAllenKey.html">2.5mm ball-ended hex key</a> to turn the screws.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/middle_plate_to_focus.jpg" alt="" />
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/middle_plate_to_focus_1.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show0" src="images/middle_plate_to_focus.jpg" alt=""/></div>
<h2 class="page-step" id="attach-the-small-standoffs">Step 2: Attach the small standoffs</h2>
<ul>
<li>Insert a <a href="parts/mech/nuts.html">M3 nut</a> into the nut trap of the <a href="models/standoff-M.html">standoff</a> (using the <a href="models/actuator-assembly-tools.html">nut insertion tool</a>). Do the same for the four standoffs.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show1').src=this.src" src="images/standoff-screw.jpg" alt="" />
<img onmouseover="getElementById('gallery-show1').src=this.src" src="images/standoff-screw_1.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show1" src="images/standoff-screw.jpg" alt=""/></div>
<ul>
<li>Insert a <a href="parts/mech/M3-15.html">M3x15mm cap head screw</a> into each small <a href="models/standoff-S.html">standoff</a> using the large hole that comes out the side of the <a href="models/standoff-S.html">standoff</a>. The screw should poke out the end of the part as shown.</li>
<li>Use a <a href="parts/tools/2.5mmBallEndAllenKey.html">2.5mm ball-ended hex key</a> to turn the screw.</li>
</ul>
<p><img alt="" src="images/standoff-M.jpg" title="" /></p>
<ul>
<li>Attach each small <a href="models/standoff-S.html">standoff</a> to one of the corners of the <a href="parts/materials/middle-plate.html">middle plate</a>.</li>
<li>Use the <a href="models/actuator-assembly-tools.html">nut insertion tool</a> and the <a href="parts/tools/2.5mmBallEndAllenKey.html">2.5mm ball-ended hex key</a> to secure the other three parts.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show2').src=this.src" src="images/standoff-M-attached.jpg" alt="" />
<img onmouseover="getElementById('gallery-show2').src=this.src" src="images/standoff-M-attached-1.jpg" alt="" />
<img onmouseover="getElementById('gallery-show2').src=this.src" src="images/standoff-M-attached-2.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show2" src="images/standoff-M-attached.jpg" alt=""/></div>
<hr />
<p><a href="attach-parts-to-top-plate.html">Previous page</a> | <a href="attach-parts-to-bottom-plate.html">Next page</a></p>
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<p class="author">©2024 Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="email">Contact: <a href="mailto:[email protected]">[email protected]</a></p>
<p class="license">Strobe-enhanced microscopy stage is released under <a href="license.html">CERN-OHL-S-2.0</a>.</p>
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