Seeking Advice on Seurat Mulitmodal Analysis

[Angeline-sys]

Hello! I’m using the Seurat pipeline for multimodal analysis and wanted to ensure I’m on the right track.
Here’s what I’ve done so far:
• I have both the RNA and ADT assays in my Seurat object.
• I’ve included clonotype information as metadata in my Seurat object.

My plan is to analyze the RNA expression data separately, including QC, dimensionality reduction, and clustering. For the ADT data, I’m planning to normalize and scale the data, then use the multimodal workflow from Seurat to compare RNA and protein side by side.

I would greatly appreciate any advice or tips to ensure I’m on the right track and if there’s a better approach to this analysis.

Thanks in advance!