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Version 0.3.1

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@dpryan79 dpryan79 released this 19 Mar 14:57
· 66 commits to master since this release

0.3.1

  • The various bedGraph files didn't previously have a "track" line. The UCSC
    Genome Browser requires this, so bedGraph files produced will now contain
    it. It should be noted that this is the very minimal line required. Bison
    does not provide facilities for making these changes, users need to edit
    things manually or use external programs for this. It should also be noted
    that any changes to the "track" or other header lines should be made after
    all processing with Bison is complete.
  • Add conversion scripts for import into MethylSeekR, BiSeq, and BEAT.
  • Revamped how bison_markduplicates works. The 3' coordinates are now
    ignored, soft-clipped bases on the 5' end are now incorporated in
    determining the 5' coordinate and methylation calls are also used in
    determining if reads/pairs are duplicates. This should be a much more
    robust (though more resource intensive) method than that previously used.
    Whereas the previous version kept unmarked the read/pair with the highest
    MAPQ, this one will do that for the read/pair with the highest summed phred
    score (a la picard).