Version 0.3.1
0.3.1
- The various bedGraph files didn't previously have a "track" line. The UCSC
Genome Browser requires this, so bedGraph files produced will now contain
it. It should be noted that this is the very minimal line required. Bison
does not provide facilities for making these changes, users need to edit
things manually or use external programs for this. It should also be noted
that any changes to the "track" or other header lines should be made after
all processing with Bison is complete. - Add conversion scripts for import into MethylSeekR, BiSeq, and BEAT.
- Revamped how
bison_markduplicates
works. The 3' coordinates are now
ignored, soft-clipped bases on the 5' end are now incorporated in
determining the 5' coordinate and methylation calls are also used in
determining if reads/pairs are duplicates. This should be a much more
robust (though more resource intensive) method than that previously used.
Whereas the previous version kept unmarked the read/pair with the highest
MAPQ, this one will do that for the read/pair with the highest summed phred
score (a la picard).