Scripts I used to process LSU metataxonomics and metagenomics data from human RS2 study
68 overweight [body mass index (BMI) ≥27 kg/m2] adults aged 35–75 y with prediabetes were randomized to consume 45 g/d of high-amylose maize (RS2) or an isocaloric amount of the rapidly digestible starch amylopectin (control) for 12 wk. Two fecal samples were collected from each subject at baseline and post-intervention (4 samples total) and frozen prior to DNA extraction. The 16S rRNA gene V4 region was amplified and sequenced for metataxonomics analysis. Additionally, high-quality gDNA from a subset of samples was submitted to NovoGene (https://en.novogene.com/) for metagenomic sequencing.
- National Institute of Diabetes and Digestive and Kidney Diseases grant R01DK092575
- National Center for Advancing Translational Sciences career development grant KL2TR001419
- Nutrition Obesity Research Center (NORC) grant P30DK072476
- Louisiana Clinical and Translational Science Center (LA CaTS) grant U54GM104940
- Peterson, C. M., Beyl, R. A., Marlatt, K. L., Martin, C. K., Aryana, K. J., Marco, M. L., ... & Ravussin, E. (2018). Effect of 12 wk of resistant starch supplementation on cardiometabolic risk factors in adults with prediabetes: a randomized controlled trial. The American Journal of Clinical Nutrition, 108(3), 492-501.
- Zach's paper (in progress)